Dietary diversity and its relationship with nutritional adequacy in 24 to 59 months old children in Iran: study protocol | BMC Nutrition

Developing the dietary variety questionnaire

Guided by FAO Guideline for measuring particular person dietary variety [3, 9], scale gadgets will likely be generated. According to the rule of thumb, as Table 1 reveals, meals are categorized into 9 teams. Food portions of a minimum of one tablespoon (≥ 15 g) will likely be included within the rating. Dietary variety scores will likely be calculated by summing the variety of meals teams consumed by the person respondents.

Table 1 Food groupings for calculation of dietary variety rating

Content validity

Experts in diet will consider the primary model of the questionnaire relating to its relevance, readability, meaningfulness and completeness.

Predictive validity

To analyze any correlation between DDS and NARs, imply adequacy ration (MAR), anthropometric indicators (Z scores of top for age, weight for age, and BMI for age), and serum retinol, Pearson correlation evaluation will likely be carried out.

Sample dimension

To calculate the pattern dimension, α and β errors have been thought-about to be 5% and 10%, respectively. According to Hatløy [10], the correlation coefficient between MAR and DDS was estimated to be 0.4. The pattern dimension was calculated as 51 kids in every province.

Recruitment and eligibility screening

Children aged 24–59 months from eleven provinces, together with Sistan-Baloochestan, South Khorasan, Ilam, Kohkiluyeh-Boyerahmad, Khuzestan, Kerman, Hormozgan, Bushehr, Kordestan, North Khorasan, and Tehran will likely be included on this research. Exclusion standards embrace reluctance to take part within the research, bodily and/or psychological retardation, and adherence to particular diets. Eligible kids will likely be enrolled via random cluster sampling primarily based on proportion to dimension. The Statistics Center of Iran will present the checklist of clusters. Ten clusters, together with seven city and three rural clusters, will likely be randomly chosen in every province. A complete of 561 kids will likely be enrolled.

Data assortment

We will use a cell utility and an digital questionnaire to gather data. General data will likely be obtained utilizing a questionnaire. The weight and top of the youngsters will likely be measured by normal strategies. Biochemical testing will likely be carried out for every baby Serum retinol assessments will likely be carried out in the identical laboratory utilizing high-performance liquid chromatography (HPLC). All mother and father/caregivers ought to signal an knowledgeable consent type after a full evaluation of the inclusion and exclusion standards and an evidence of the research protocol. The research protocol has been permitted by the Ethical Committees of National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences.

Nutritional evaluation

The dietary variety questionnaire will likely be accomplished by the youngsters’s mother and father/caregivers. Also, with a view to consider the consumption of power and vitamins, two 24-h meals remembers will likely be accomplished. The interval between the 2 remembers is one to a most of seven days. The consumed meals will first be transformed to grams, then the vitamins and power contents will likely be calculated utilizing the Nutritionist-IV software program and United States Department of Agriculture (USDA) meals composition desk (FCT). An Iranian FCT may also be used for native meals gadgets. All phases will likely be carried out by skilled and skilled nutritionists.

Nutrient adequacy

To estimate the nutrient adequacy, Nutrient Adequacy Ratio (NAR) for the power, protein, and micronutrients (vitamin A, vitamin E, B1, B2, niacin, B6, B12, folate, vitamin C, calcium, iron, and zinc) will likely be calculated [10]. As an general measure of nutrient adequacy, MAR will likely be calculated, which is the sum of every NAR (truncated at 100%) divided by the variety of vitamins (excluding power and protein) [10]. For each NAR and MAR, a worth of 100% is right because it implies that the consumption is similar because the requirement. The power, protein, nutritional vitamins, and minerals requirement for every intercourse and age group will likely be calculated primarily based on the Estimated Average Requirements (EAR) [11,12,13].

Data evaluation

Data will likely be analyzed utilizing STATA software program (model 17; StataCorp). A P worth < 0.05 will likely be thought-about statistically important for all analyses. To analyze anthropometric knowledge, the WHO Anthro Survey Analyzer will likely be utilized. The knowledge file in a comma-delimited format (.csv) will likely be uploaded to the URL: After calculating DDS, to research any correlation between DDS and NARs, MAR, anthropometric indicators (Z scores of top for age, weight for age, and BMI for age), and serum retinol, Pearson correlation evaluation will likely be carried out.

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